By Clive Robinson (auth.), Eric Reid, J. D. Robinson, Ian D. Wilson (eds.)
vi as did non-appreciation that % values for bought-in suggestions (notably ammonia) could be on a weight foundation, no longer made glaring via the producer. although the shortcomings or lateness of a few texts, authors are thanked for compiling them amidst different pressures. Elsevier and the yank Chemical Society also are thanked, for Figures now reproduced with resource acknowledgement. This Editor has as a rule revered authors' phraseology, when shuddering while the time period 'incubate' is encountered in a nil° context. He continues to be a 'diehard' in yes respects, particularly in favouring 'M' instead of 'mol/I', and a wt./ml foundation for drug concentrations in try samples; he regards 'mmol/l' as a fatuous style. referring to infelicitous abbreviations, a contrast is made among electron seize (detector context; 'ECD') and electrochemical ('EC', by no means 'ECD'); the hallowed GC time period 'FID' potential unfastened induction decay to NMR practi tione:ts, who might pardon the time period 'Fid' as brought editorially. The conference for ,0C' during the e-book is '0'. Undefined yet famous abbreviations comprise GC, HPLC and TLC. MS (mass spectrometry), NPD (nitrogen-phosphorus detector), tr (retention time) and RIA (radioimmunoassay) tend to be outlined within the article involved, as are the HPLC modes NP (normal-/straight part) and RP (reversed-phase; C-lS and ODS are synonymous), and i.s.
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Extra info for Bioanalysis of Drugs and Metabolites, Especially Anti-Inflammatory and Cardiovascular
Holler, in preparation). Wolfe et a1.  determined PGF 2Q and PGE 2 in rat brain by GC-MS, and obtained values in the same range as the present ones. Brain levels of TXB 2 and 6-keto-PGF J in the rat were determined by RIA after microwave fixation or decapitation . The present results are -30-50% higher for both compounds than in their microwave fixation group and are considerably lower than in the decapitation group. CONCLUDING COMMENTS With the method presented here, PGD 2 , PGE 2 , PGF 2Q , 6-keto-PGF 1 and TXB 2 can easily be determined in non-ischaemic brain.
This is of low intensity and sometimes absent from the EI mass spectra. However, these derivatives show characteristic fragmentations which allmv the assignment of sites of metabolism (Fig. 3). W. J0 PFB . ,0 o Si(Me)3 ~ /~O M-PFBl- OMe Fig. 3. Key fragmentation pathways for (A) ME-MO-TMS Gerivatives, and (B) PFB-MO-TMS derivatives. From ref. f6}, by permission. conditions >-lith ammonia as the reagent gas. Essentially little or no fragmentation is seen in the mass spectra or these derivatives.
The eluate was dried down and the residue subjected to methoximation, PFB esterification and silylation. The derivatized COP's were separated and quantitated by NCI GC-MS. 5 pg per column injection of standards for each COP. For determination in brain tissue the limit was 100 pg per sample. 2-50 ng/50 mg dry tissue were linear for each COP. The method is suitable for determining various PG's and TXB 2 in the normoxic brain. In ischaemia of the brain, activation of phospholipases leads to liberation of arachidonic acid with subsequent formation of COP's tI,2].
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